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What are Gram positive and Gram negative bacteria ?

What are bacteria ?

  • Bacteria are procaryotes that are usually single-celled organisms.
  • Most bacteria  have cell walls that contain the structural molecule peptidoglycan.
  •  They are found in soil, water, and air and are also major inhabitants of our skin, mouth, and intestines. 
  • Some bacteria live in environments that have extreme temperatures, pH, sanlinity.
  • some bacteria cause disease, many play more beneficial roles such as cycling elements in the biosphere,breaking down dead plant and animal material, and producing vitamins.

What are Gram positive bacteria ?

Gram positive bacteria are bacteria that stains purple in colour after gram staining which indicates a positive result, it helps to classify bacteria on the basis of their cell wall.

Below is the examples of some Gram positive bacteria:

What are Gram negative bacteria ?

Gram negative bacteria are bacteria which gives negative results by retaining the gram stain ,which gives pink colour after gram staining. Gram negative bacterial cell wall retain gram stain which helps to differentiate the two types of bacteria.

Below is the examples of Gram negative bacteria :

Difference Between Gram positive and Gram negative bacteria :






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Gram staining | History | Application |Principle | Procedure

Gram Stain In 1884, Hans Christian Gram discovered a staining technique that could help to visualise the infectious bacteria clearly and can easily study their morphology.   In this technique he used   crystal violet (the primary dye),  Gram’s iodine (IKI, the mordant),  an alcohol rinse (decolorizer),   Safranin (counter stain ). A Gram stain is a test that checks for bacteria at the site of a suspected infection such as the throat, lungs, genitals, or in skin wounds.  Gram stains may also be used to check for bacteria in certain body fluids, such as blood or urine . This color choice provides differentiation between bacteria that stain purple, called gram-positive, and those that stain red, called gram-negative. The difference between gram-positive and gram-negative bacteria is due to the physical nature of their cell walls and how it reacts to the   series of reagents applied to the cells . Gram positive bacteria has thick peptidoglycan layer in the cell wall and gram bacteria